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Lakshmi Narsu, M.
- Optimisation Studies for the Immobilization of Oligonucleotide Probe on Silica Nanoparticles
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1 Department of Biotechnology, J.N.T.U.A. College of Engineering, Pulivendula-516390 Andhra Pradesh, IN
2 Department of Biotechnology, J.N.T.U, Hyderabad-500085, Andhra Pradesh, IN
1 Department of Biotechnology, J.N.T.U.A. College of Engineering, Pulivendula-516390 Andhra Pradesh, IN
2 Department of Biotechnology, J.N.T.U, Hyderabad-500085, Andhra Pradesh, IN
Source
International Journal of Advanced Biotechnology Research, Vol 3, No 1 (2013), Pagination: 9-17Abstract
Bionanotechnology refers to engineered nanostructures and nanomachines or naturally occurring cell machinery with biological molecules such as proteins, peptides, DNA, RNA in a living organism. A new approach for immobilization of probe oligonucleotides that uses 3- Aminopropyltriethoxysilane modified silica nanoparticles is proposed. Various studies, i.e., synthesis of silica nanoparticles, their surface modification, probe immobilization, effect of time and fluorescence measurement of hybridization were carried out during optimization of reaction conditions. The probe modified silica nanoparticles were retained their properties and the hybridization was induced by exposure of single-stranded DNA (ssDNA) containing silica nanoparticles to the complementary DNA in solution. The decrease in the fluorescence signal for two mismatch, three mismatch and 50% DNA was observed upon hybridization of probe with target DNAs, while there was no response for the random target ssDNA under the same experimental conditions. This hybridization assay is simple and possesses good analytical characteristics which can provide an effective and efficient method for development of DNA biosensors and biochips.Keywords
Silica Nanoparticles, Tetraethyl Orthosilicate (TEOS), Immobilization, Single-Stranded Deoxyribonucleic Acid (ssDNA), HybridizationReferences
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